골다공증 유도 흰쥐에서 칼슘과 비타민 D 섭취가 티타늄 임플랜트 주위의 골 형성에 미치는 영향
Effect of calcium and vitamin D supplementation on bone formation around titanium implant in osteoporosis-induced rats
이재열, 정석영, 신상훈, 김규천, 김용덕, 정인교, 김욱규,
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이재열 ( Lee Jae-Yeol ) - 부산대학교 치의학전문대학원 구강악안면외과학교실
정석영 ( Jeong Seok-Young ) - 부산대학교 치의학전문대학원 구강악안면외과학교실
신상훈 ( Shin Sang-Hun ) - 부산대학교 치과대학 구강안면외과학교실
김규천 ( Kim Gyoo-Cheon ) - 부산대학교 치의학전문대학원 구강해부학교실
김용덕 ( Kim Yong-Deok ) - 부산대학교 치의학전문대학원 구강악안면외과학교실
정인교 ( Jung In-Kyo ) - 부산대학교 치과대학 구강안면외과학교실
김욱규 ( Kim Uk-Kyu ) - 부산대학교 치과대학 구강악안면외과학교실
KMID : 0355620080340030276
Abstract
Purpose:The purpose of this study was to observe the effect of calcium and vitamin D to the titanium implant osseointegration in the osteoporosis-induced animal model.
Material and Methods :Thirty-two rats, 10 weeks of age, were divided into two groups: experimental group was ingested additional calcium and vitamin D , and a control group was not. Titanium screw implant(diameter, 2.0 mm; length, 3.5 mm; pitch-height 0.4 mm) were placed into tibia of 32 rats, 16 in the control group and 16 in the experimental group. The rats were sacrificed at 1, 2, 4 and 8 weeks after implantation for histopathologic examination, histomorphometric analysis and immunohistochemistry with fibronectin and collagen type I antibody.
Results:In histopathological findings, newly formed bone was seen at 2 weeks and became lamellar bone at 4 weeks, and mature trabecullar bone was seen at 8 weeks in experimental group. In control group, thickness of regenerated bone increased till 4 weeks gradually and trabecullar bone was seen at 8 weeks. In histomorphometric analysis, marrow bone density increased significantly in experimental group compared to control group. Fibronectin immunoreactivity was strong at 2 weeks in experimental group and reduced after 4 weeks gradually. But it was maintained continuously from 2 to 8 weeks in control group. Collagen type I immunoreactivity was very strong from 2 to 4 week in experimental group. And the amount of Collagen type I expression was more abundant in experimental group.
Conclusion: The results of this study suggest that calcium and vitamin D supplementation promote bone healing around titanium implants in osteoporosis induced animals.
키워드
Osteoporosis; Implant;Calcium;Vitamin D
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